f19d7d77-7a9e-498a-a5ca-2b37efe2b06b20221027062818636naun:naunmdt@crossref.orgMDT DepositInternational Journal of Systems Applications, Engineering & Development2074-130810.46300/91015http://www.naun.org/cms.action?id=3100111202211120221610.46300/91015.2022.16https://npublications.com/journals/saed/2022.phpSriAndayaniBrawijaya University, Faculty of Fisheries and Marine Science, Aquaculture Department, Malang, 65145, East Java, IndonesiaHenySuprastyaniBrawijaya University, Faculty of Fisheries and Marine Science, Aquaculture Department, Malang, 65145, East Java, IndonesiaAuliaRahmawatiBrawijaya University, Faculty of Fisheries and Marine Science, Aquaculture Department, Malang, 65145, East Java, IndonesiaM. SulaimanDadionoJendral Sudirman University, Faculty of Fisheries and Marine Science, Aquaculture Department, Banyumas, 53122, Central Java, IndonesiaWidya TriElwiraBrawijaya University, Faculty of Fisheries and Marine, Postgraduate Program, Aquculture Department, Malang, 65145, East Java, IndonesiaThis study aims to analyze the blood glucose of koi fish exposed to polyphenol fraction of A. vera. It firstly found LC50 at the treatment of 150 mg.kg-1 that caused 50% mortality, so that the doses of A. vera fraction injected into the fish muscle as follows: Control (-) no treatment, Control (+) was given tannin/synthetic polyphenolic compound as much as 3 mg.kg-1 of body weight, treatment A= 75 mg.kg-1 of body weight, B= 100mg.kg-1 of body weight, C= 125 mg.kg-1 of body weight, respectively. After 72 h of injection, the blood plasm of Koi fish was taken to observe the stress effect on the blood glucose. Results showed that for blood chemistry, glucose with increased polyphenol fraction application due to higher stress level, with increased polyphenol dose. The clinical symptom after injected with polyphenol fraction of A. vera resulted in abnormal swimming, red spots, paleness, and hemorhage.1027202210272022919616https://npublications.com/journals/saed/2022/a322014-016(2022).pdf10.46300/91015.2022.16.16https://npublications.com/journals/saed/2022/a322014-016(2022).pdfDirectorate General of Aquaculture, Annual report of production of 2019. Jakarta: Direktorat Jenderal Perikanan Budidaya, 2019. 10.1016/0141-1136(95)00015-1H. Roche and G. Boge, “Fish blood parameters as a potential tool for identification of stress caused by environmental factors and chemical intoxication,” Marine Environmental Research, vol. 41, no. 1, pp. 27-43, 1996. 10.1016/s0041-0101(97)00020-2H. Avila, J. Rivero, F. Herrera, and G. 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